Whole Exome Sequencing

Service icon Our group has delivered on demanding exome projects from those involving hundreds of samples down to unique cases requiring exomes from only 100ng of DNA.

Although we specialize in exome library construction from human cancer samples, we have in-house expertise with many types of samples all the way from abundant cell lines all the way to the most degraded archival specimens you can find.

Card image cap

Capabilities

Service Applications Description
Standard Exomes
  • Agilent XT2, XT platform
  • NovaSeq or HiSeq 2500 sequencing
  • Mean coverage 50X, 80% of target >20X
  • >100ng of intact or FFPE DNA required
Used for detecting variants across a variety of biomedical genomics studies, our standard exomes provide 30X or greater coverage across 80% of targeted regions (50X MTC). We carry many of Agilent's SureSelect exome designs in stock and can match the right set of capture reagents to your application. Aligned BAM files are available with a 6-8 week turnaround time.
Deep Exomes
  • Agilent XT2, XT platform
  • NovaSeq or HiSeq 2500 sequencing
  • Mean coverage 200X, 80% of target >50X
  • >100ng of intact or FFPE DNA required
Deep exomes are used for detecting lower frequency variants in cancer studies interested in finding clones down to 1-3% abundance. The library preparation methods for deep exomes are the same as for standard exomes, but we recommend smaller exome designs to focus sequencing reads on regions of interest thereby keeping project costs lower. Aligned BAM files are available with a 6-8 week turnaround time.
Custom Exomes
  • Various platforms
  • NovaSeq or HiSeq 2500 sequencing
  • Any coverage requirements
  • As little as 50ng of DNA required
Some projects have needs outside of our standard human exome pipeline. We've completed projects with various exome panels, types of sample, or sample input amounts. We encourage you to contact us to discuss the requirements of your specific project.