Single Cell Sequencing

Service icon Single Cell Sequencing from cell suspensions, solid tissue samples, and other unique samples in the heart of Toronto's Discovery District

OICR's team has provided research groups with 10X Genomics' single cell transcriptomes, linked-reads and full-length paired V(D)J transcripts from 100s to 1000s of cells using the 10X Genomics Chromium technology.

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Capabilities

Service Applications Description
Single Cell Transcriptome Solution
  • Tumour/Cell line heterogeneity studies
  • Detection of rare subpopulations
  • Drug response studies
10X Genomics high-throughput scRNA-seq technology provides single cell expression measurements that enable discovery of gene expression dynamics and molecular profiling of individual cell types a. Offer complete workflow starting from a solution of 100s to 1000s of cells to sequencing. Contact us for tissue dissociation solutions. b. flexible bioinformatics approaches: FASTQ files, primary Cell Ranger analysis for single cell gene expression, custom secondary analyses
Single Cell V(D)J Solution
  • Single cells immune profiling
  • Immune response studies
Enables assembly of full-length V(D)J sequences on a cell-by-cell basis, providing high resolution insights into the adaptive immune system a. Offer complete workflow starting from a solution of 100s to 1000s of cells to sequencing b. Flexible bioinformatics approaches: FASTQ files, primary Cell Ranger analysis for full-length V(D)J sequences, custom secondary analyses

Resources

This technical note describes best practices for sample preparation leading into 10X single cell gene expression experiments, as well as other considerations that influence experimental design of scRNA-seq experiments. The impact of sample quality on downstream bioinformatic analysis is also shown.