OICR's team has provided research groups with 10X Genomics' single cell transcriptomes, linked-reads and full-length paired V(D)J transcripts from 100s to 1000s of cells using the 10X Genomics Chromium technology.
|Single Cell Transcriptome Solution||
||10X Genomics high-throughput scRNA-seq technology provides single cell expression measurements that enable discovery of gene expression dynamics and molecular profiling of individual cell types a. Offer complete workflow starting from a solution of 100s to 1000s of cells to sequencing. Contact us for tissue dissociation solutions. b. flexible bioinformatics approaches: FASTQ files, primary Cell Ranger analysis for single cell gene expression, custom secondary analyses|
|Single Cell V(D)J Solution||
||Enables assembly of full-length V(D)J sequences on a cell-by-cell basis, providing high resolution insights into the adaptive immune system a. Offer complete workflow starting from a solution of 100s to 1000s of cells to sequencing b. Flexible bioinformatics approaches: FASTQ files, primary Cell Ranger analysis for full-length V(D)J sequences, custom secondary analyses|
|Interested in any of these services? work with us|
This technical note describes best practices for sample preparation leading into 10X single cell gene expression experiments, as well as other considerations that influence experimental design of scRNA-seq experiments. The impact of sample quality on downstream bioinformatic analysis is also shown.